Workflows
Last Updated: January 27, 2025
Overview
The AI Workspace offers two distinct workflow types for analyzing single-cell RNA sequencing data. Each workflow serves different analytical needs and has specific requirements.
Standard Workflow
Analyze your dataset independently without reference to external data.
- Purpose: Single-dataset analysis and exploration
- Data Requirements: H5AD files with ENSEMBL gene IDs
- Available Models: PCA, scVI, TranscriptFormer
- Organism Support: Varies by model (see Data Requirements)
Learn more about Standard Workflow →
Comparison Workflow
Compare your data against the CELLxGENE Census reference atlas.
- Purpose: Contextualize cells against comprehensive reference data
- Data Requirements: H5AD files with human or mouse data only
- Available Models: scVI, TranscriptFormer only
- Gene Requirements: ENSEMBL IDs (ENSG or ENSMUSG prefixes)
Learn more about Comparison Workflow →
Cell Type Classification
Automated cell type annotation available for both workflows when using compatible models and organisms.
- Requirements: scVI or TranscriptFormer models with human or mouse data
- Output: Predicted cell type labels with confidence scores
- Integration: Available as optional configuration in both workflows
Workflow Selection
Feature | Standard | Comparison |
|---|---|---|
Organism Support | Model-dependent | Human/mouse only |
Available Models | PCA, scVI, TranscriptFormer | scVI, TranscriptFormer |
Data Requirements | Raw or processed (model-dependent) | Raw integer counts only |
Reference Integration | None | CELLxGENE Census |
| Cell Type Classification | Only with scVI or TranscriptFormer (human/mouse) | Only with scVI or TranscriptFormer (human/mouse) |