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Workflows

Last Updated: January 27, 2025

Overview

The AI Workspace offers two distinct workflow types for analyzing single-cell RNA sequencing data. Each workflow serves different analytical needs and has specific requirements.

Standard Workflow

Analyze your dataset independently without reference to external data.

  • Purpose: Single-dataset analysis and exploration
  • Data Requirements: H5AD files with ENSEMBL gene IDs
  • Available Models: PCA, scVI, TranscriptFormer
  • Organism Support: Varies by model (see Data Requirements)

Learn more about Standard Workflow →

Comparison Workflow

Compare your data against the CELLxGENE Census reference atlas.

  • Purpose: Contextualize cells against comprehensive reference data
  • Data Requirements: H5AD files with human or mouse data only
  • Available Models: scVI, TranscriptFormer only
  • Gene Requirements: ENSEMBL IDs (ENSG or ENSMUSG prefixes)

Learn more about Comparison Workflow →

Cell Type Classification

Automated cell type annotation available for both workflows when using compatible models and organisms.

  • Requirements: scVI or TranscriptFormer models with human or mouse data
  • Output: Predicted cell type labels with confidence scores
  • Integration: Available as optional configuration in both workflows

Workflow Selection

Feature

Standard

Comparison

Organism Support

Model-dependent

Human/mouse only

Available Models

PCA, scVI, TranscriptFormer

scVI, TranscriptFormer

Data Requirements

Raw or processed (model-dependent)

Raw integer counts only

Reference Integration

NoneCELLxGENE Census
Cell Type Classification

Only with scVI or TranscriptFormer (human/mouse)

Only with scVI or TranscriptFormer (human/mouse)